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ASCP-MLT Questions and Answers

Question # 1

Conversion of only the slant to a pink color in a Christensen's urea agar slant is produced by bacterial species that have weak urease activity. The reaction in the slant to the right is often produced by Klebsiella species, as an example. Strong urease activity is indicated by conversion of the slant and the butt of the tube to a pink color, as seen in the tube to the left. The slant only reaction in the right tube may be seen early on if only the slant had been inoculated; however, with a strong urease producer, both the slant and the butt would turn. Therefore, the reaction is dependent on the strength of urease activity. If the media had outdated for a prolonged period, either there would be no reaction or the appearance of only a faint pink tinge, either in the slant, the butt or both, again depending on the strength of urease production by the unknown organism.

The urease reaction seen in the Christensen's urea agar slant on the far right indicates:

Weak activity

Strong activity

Slant only inoculated

Use of outdated medium

Question # 2

Immunoassay is the most common technique that is used by clinical laboratories for therapeutic drug monitoring.

Most of the drugs commonly assessed with TDM can be measured on analytical platforms which utilize antibodies (in some form) for detection. Antibodies can be developed that recognize drugs. Although most drugs are much too small to evoke an immune response, scientists can conjugate drugs to immunogenic proteins to produce antibodies that recognize drug-specific epitopes.

Which of the following is the most common technique that is used by clinical laboratories for therapeutic drug monitoring?

Immunoassay

Electrophoresis

Atomic absorption

Ion selective electrode voltimetry

Question # 3

Patients with antibody to the following antigen are immune to Hepatitis B:

Core antigen

Surface antigen

antigen

Delta antigen

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